Application Data
(Recombinant human SERPINC1 inhibits Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is )
Serpin C1/Antithrombin III/ATIII Active Protein | ATIII active protein
Recombinant Human Serpin C1/Antithrombin III/ATIII Protein
Gene Names
SERPINC1; AT3; AT3D; ATIII; THPH7; ATIII-R2; ATIII-T1; ATIII-T2
Purity
>95% by SDS-PAGE.
Synonyms
Serpin C1/Antithrombin III/ATIII; N/A; Recombinant Human Serpin C1/Antithrombin III/ATIII Protein; SerpinC1; AntithrombinIII; ATIII; ATIII active protein
Host
HEK293 Cells
Purity/Purification
>95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Sequence Length
464
Tag
C-His
Species
Human
Endotoxin
< 0.1 EU/ug of the protein by LAL method.
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Bio-Activity
Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <0.48 nM
Preparation and Storage
Store at -20°C.
Store the lyophilized protein at -20 to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Avoid repeated freeze/thaw cycles.
Store the lyophilized protein at -20 to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Avoid repeated freeze/thaw cycles.
Application Data
(Recombinant human SERPINC1 inhibits Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is )
Application Data
(Recombinant human SERPINC1 inhibits Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is )
SDS-PAGE
(Recombinant Human Serpin C1/Antithrombin III/ATIII Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55-65 kDa.)
SDS-PAGE
(Recombinant Human Serpin C1/Antithrombin III/ATIII Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55-65 kDa.)
Related Product Information for ATIII active protein
Description: Recombinant Human SerpinC1/AntithrombinIII/ATIII Protein is produced by HEK293 expression system. The target protein is expressed with sequence (His33-Lys464) of human SerpinC1/AntithrombinIII/ATIII (Accession #NP_000479.1) fused with a 6xHis tag at the C-terminus.
Background: The protein is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
Background: The protein is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
Product Categories/Family for ATIII active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Antithrombin-III
NCBI Official Synonym Full Names
serpin family C member 1
NCBI Official Symbol
SERPINC1
NCBI Official Synonym Symbols
AT3; AT3D; ATIII; THPH7; ATIII-R2; ATIII-T1; ATIII-T2
NCBI Protein Information
antithrombin-III
UniProt Protein Name
Antithrombin-III
UniProt Gene Name
SERPINC1
UniProt Synonym Gene Names
AT3; ATIII
UniProt Entry Name
ANT3_HUMAN
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Product Notes
The ATIII serpinc1 (Catalog #AAA281508) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Serpin C1/Antithrombin III/ATIII, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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