Human Serpin C1/Antithrombin-III PicoKine ELISA Kit | SERPINC1 elisa kit

Human Serpin C1/Antithrombin-III PicoKine ELISA Kit

Cat. Num. AAA46157

• Gene Names: SERPINC1; AT3; AT3D; ATIII; THPH7
• Reactivity: Human
• Synonyms: Serpin C1/Antithrombin-III PicoKine; N/A; Human Serpin C1/Antithrombin-III PicoKine ELISA Kit; Antithrombin-III; ANT3_HUMAN; Antithrombin; Antithrombin III; AntithrombinIII; AT 3; AT III; AT3; AT3D; ATIII; Heparin cofactor I; MGC22579; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1; Serine cysteine proteinase inhibitor clade C member 1; Serine proteinase inhibitor clade C member 1; Serpin C1; Serpin peptidase inhibitor clade C (antithrombin) member 1; SERPINC1; THPH7 ; serpin peptidase inhibitor, clade C (antithrombin), member 1; SERPINC1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Serpin C1
• Sequence Length: 464
• Assay Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human Serpin C1 in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.

• Immunogen: NSO, H33-K464
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20,000pg/ml, 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|1.05|3.42|8.67|1.32|3.74|8.23; Standard deviation|0.064|0.194|0.546|0.088|0.239|0.543; CV(%)|6.1|5.7|6.3|6.7|6.4|6.6
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10,000|20,000; O.D.|0.011|0.062|0.082|0.15|0.295|0.582|1.333|1.974
• Anticoagulant: Heparin, EDTA or Citrate
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human Serpin C1/Antithrombin-III PicoKine ELISA Kit standard curve)

Related Product Information for SERPINC1 elisa kit

Principle of the assay: Human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.

Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).

NCBI and Uniprot Product Information

• NCBI GI #: 4502261
• NCBI GeneID: 462
• NCBI Accession #: NP_000479.1
• NCBI GenBank Nucleotide #: NM_000488.3
• UniProt Accession #: P01008; P78439; P78447; Q13815; Q5TC78; Q7KZ43; Q7KZ97; Q9UC78; B2R6P0
• Molecular Weight: 52,602 Da
• NCBI Official Full Name: antithrombin-III
• NCBI Official Synonym Full Names: serpin family C member 1
• NCBI Official Symbol: SERPINC1
• NCBI Official Synonym Symbols: AT3; AT3D; ATIII; THPH7
• NCBI Protein Information: antithrombin-III
• UniProt Protein Name: Antithrombin-III
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; ATIII
• UniProt Entry Name: ANT3_HUMAN