Carbonic Anhydrase IV Cell Lysate | CA4 cell lysate
Mouse Carbonic Anhydrase IV HEK293 Overexpression Lysate
Gene Names
Car4; Ca4; AW456718
Applications
Western Blot
Synonyms
Carbonic Anhydrase IV; N/A; Mouse Carbonic Anhydrase IV HEK293 Overexpression Lysate; Mouse Carbonic Anhydrase IV/Car4 HEK293 Cell Lysate (WB positive control); carbonic anhydrase IV; Mouse AW456718 Overexpression Lysate; Mouse Ca4 Overexpression Lysate; Mouse Car4 Overexpression Lysate; Mouse RP23-167D6.1 Overexpression Lysate; CA4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
271aa
Sequence
Met1-Ser277
Applicable Applications for CA4 cell lysate
WB (Western Blot)
Predicted N Terminal
Glu 18
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Preparation and Storage
Store at 4°C. After re-dissolution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles.
Related Product Information for CA4 cell lysate
Human Cell lysate that Mouse Car4 / CA4 / CAIV transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
The secreted recombinant mouse Car4 consists of 271 amino acids and has a calculated molecular mass of 31 kDa as estimated in SDS-PAGE under reducing conditions.
NCBI Official Full Name
carbonic anhydrase 4
NCBI Official Synonym Full Names
carbonic anhydrase 4
NCBI Official Symbol
Car4
NCBI Official Synonym Symbols
Ca4; AW456718
NCBI Protein Information
carbonic anhydrase 4
UniProt Protein Name
Carbonic anhydrase 4
UniProt Gene Name
Ca4
UniProt Synonym Gene Names
Car4; CA-IV
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Product Notes
The CA4 ca4 (Catalog #AAA257383) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 271aa. AAA Biotech's Carbonic Anhydrase IV can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CA4 ca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser27 7. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IV, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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