Application Data
(Fig 2: Immobilized Recombinant 2019-nCoV Spike S1-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.)
COVID 19 Spike S1 Coronavirus Active Protein | COVID-19 active protein
Recombinant SARS-CoV-2 Spike S1 Protein with His tag and Avi
Applications
Functional Assay
Purity
>95% by SDS-PAGE.
Synonyms
COVID 19 Spike S1 Coronavirus; N/A; Recombinant SARS-CoV-2 Spike S1 Protein with His tag and Avi; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1; COVID-19 active protein
Host
HEK293 cells
Purity/Purification
>95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22um filtered solution of PBS, pH7.4
Applicable Applications for COVID-19 active protein
FA (Functional Assay)
Endotoxin
<1.0EU/ug of the protein by LAL method.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1-His at 2 ug/mL (100 ul/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.
Amino Acid note
The target protein is expressed with sequence (Gln14-Arg683) of 2019-ncov Spike S1 fused with a 6xHis tag and Avi at the C-terminus.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
Application Data
(Fig 2: Immobilized Recombinant 2019-nCoV Spike S1-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.)
Application Data
(Fig 2: Immobilized Recombinant 2019-nCoV Spike S1-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.)
SDS-PAGE
(Fig 1: Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
SDS-PAGE
(Fig 1: Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Related Product Information for COVID-19 active protein
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Categories/Family for COVID-19 active protein
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Product Notes
The COVID-19 (Catalog #AAA78533) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Spike S1 Coronavirus can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S1 Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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