TIMP-1 Active Protein | TIMP1 active protein

TIMP-1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257954

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP
• Purity: > 97% as determined by SDS-PAGE
• Synonyms: TIMP-1; N/A; TIMP-1 Protein, Human, Recombinant (His Tag); Human TIMP-1/TIMP1 Protein (His Tag); CLGI Protein, Human; EPA Protein, Human; EPO Protein, Human; HCI Protein, Human; TIMP Protein, Human; TIMP-1 Protein, Human; TIMP metallopeptidase inhibitor 1; TIMP1 active protein

• Host: HEK293 Cells
• Purity/Purification: > 97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose, 5% mannitol and 0.01% Tween80
• Sequence: Met1-Ala207

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TIMP1 (NP_003245.1)(Met 1-Ala 207) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: Reconstituted with sterile deionized water to 0.25 mg/ml
• Activity: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 . The IC50 value is <6nM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for TIMP1 active protein

Background: TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.

References

Hornebeck W (2004). Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival.. Pathol. Biol. 51 (10): 569-73.Soini Y, et al. (2001) Expression of MMP2, MMP9, MT1-MMP, TIMP-1, and TIMP2 mRNA in valvular lesions of the heart. J Pathol. 194(2):225-31.Wang X, et al. (1999) Analysis of coding sequences for tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP2) in patients with aneurysms. Matrix Biol. 18(2):121-4.

NCBI and Uniprot Product Information

• NCBI GI #: 4507509
• NCBI GeneID: 7076
• NCBI Accession #: NP_003245.1
• NCBI GenBank Nucleotide #: NM_003254.2
• UniProt Accession #: P01033; Q14252; Q9UCU1
• Molecular Weight: 22.16 kDa
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP
• NCBI Protein Information: metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN