VEGF C Active Protein | VEGFC active protein

VEGF C Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257893

• Gene Names: VEGFC; VRP; Flt4-L
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VEGF C; N/A; VEGF C Protein, Human, Recombinant (His Tag); Human VEGF-C Protein (His Tag); Flt4-L Protein, Human; LMPH1D Protein, Human; VEGF-C Protein, Human; VRP Protein, Human; vascular endothelial growth factor C; VEGFC active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr103-Arg227

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 103
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human VEGFC (NP_005420.1)(Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2ug/mL (100uL/well) can bind VEGFR3 hFc. The EC50 of VEGFR3 hFc is 2-15ng/mL.; 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4nM.; 3. Measured in a cell proliferation assay using humanuMbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.05-0.2ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 ug/mL (100 uL/well) can bind VEGFR3-His, Biotinylated, the EC50 of VEGFR3-His, Biotinylated is 1-10 ng/mL.)

SDS-PAGE

Related Product Information for VEGFC active protein

Background: Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

Product Categories/Family for VEGFC active protein

Cancer Drug Targets

References

Joukov V, et al. (1997) Vascular endothelial growth factors VEGF-B and VEGF-C. J Cell Physiol. 173(2): 211-5.Su JL, et al. (2007) The role of the VEGF-C/VEGFR-3 axis in cancer progression. Br J Cancer. 96(4): 541-5.Anisimov A, et al. (2009) Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle. Circ Res. 104(11): 1302-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.3
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L
• NCBI Protein Information: vascular endothelial growth factor C; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN