Standard Curve (Sample)
Goat Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit | HER-2 ECD elisa kit
Goat Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Goat
Synonyms
Epidermal Growth Factor Receptor 2 Extracellular Domain; N/A; Goat Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit; HER-2 ECD elisa kit
Reactivity
Goat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HER-2 ECD elisa kit
Intended Uses: This HER-2 ECD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat HER-2 ECD. This ELISA kit for research use only!
Principle of the Assay: HER-2 ECD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HER-2 ECD antibody and an HER-2 ECD-HRP conjugate. The assay sample and buffer are incubated together with HER-2 ECD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HER-2 ECD concentration since HER-2 ECD from samples and HER-2 ECD-HRP conjugate compete for the anti-HER-2 ECD antibody binding site. Since the number of sites is limited, as more sites are occupied by HER-2 ECD from the sample, fewer sites are left to bind HER-2 ECD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HER-2 ECD concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HER-2 ECD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HER-2 ECD antibody and an HER-2 ECD-HRP conjugate. The assay sample and buffer are incubated together with HER-2 ECD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HER-2 ECD concentration since HER-2 ECD from samples and HER-2 ECD-HRP conjugate compete for the anti-HER-2 ECD antibody binding site. Since the number of sites is limited, as more sites are occupied by HER-2 ECD from the sample, fewer sites are left to bind HER-2 ECD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HER-2 ECD concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HER-2 ECD elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,253 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform a
NCBI Official Synonym Full Names
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The Goat HER-2 ECD erbb2 (Catalog #AAA91214) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA91214 ELISA Kit recognizes Goat HER-2 ECD. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Receptor 2 Extracellular Domain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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