Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit | HER 2 ECD elisa kit

Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit

Cat. Num. AAA16347

• Gene Names: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Reactivity: Human
• Synonyms: Epidermal Growth Factor Receptor 2 Extracellular Domain; N/A; Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA Kit; HER 2 ECD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGFR2ED. No significant cross-reactivity or interference between EGFR2ED and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EGFR2ED and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HER 2 ECD elisa kit

Principle of the Assay: EGFR2ED ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EGFR2ED. Standards or samples are then added to the microtiter plate wells and EGFR2ED if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EGFR2ED present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EGFR2ED are added to each well to "sandwich" the EGFR2ED immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGFR2ED and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFR2ED concentration in each sample is interpolated from this standard curve.

Intended Uses: This EGFR2ED ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EGFR2ED. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 54792096
• NCBI GeneID: 2064
• NCBI Accession #: NP_004439.2
• NCBI GenBank Nucleotide #: NM_004448.3
• Molecular Weight: 97,253 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2 isoform a
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• UniProt Gene Name: ERBB2
• UniProt Synonym Gene Names: HER2; MLN19; NEU; NGL; MLN 19
• UniProt Entry Name: ERBB2_HUMAN