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ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse HIF1 alpha Polyclonal Antibody | anti-HIF1A antibody
HIF1 alpha Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Peptide affinity purified
Synonyms
HIF1 alpha, Antibody; HIF1 alpha Antibody; ARNT interacting protein antibody; ARNT-interacting protein antibody; Basic helix loop helix PAS protein MOP1 antibody; Basic-helix-loop-helix-PAS protein MOP1 antibody; bHLHe78 antibody; Class E basic helix-loop-helix protein 78 antibody; HIF 1A antibody; HIF 1alpha antibody; HIF-1-alpha antibody; HIF1 A antibody; HIF1 Alpha antibody; HIF1 antibody; HIF1-alpha antibody; HIF1A antibody; HIF1A_HUMAN antibody; Hypoxia inducible factor 1 alpha antibody; Hypoxia inducible factor 1 alpha isoform I.3 antibody; Hypoxia inducible factor 1 alpha subunit antibody; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor) antibody; Hypoxia inducible factor1alpha antibody; Hypoxia-inducible factor 1-alpha antibody; Member of PAS protein 1 antibody; Member of PAS superfamily 1 antibody; Member of the PAS Superfamily 1 antibody; MOP 1 antibody; MOP1 antibody; PAS domain-containing protein 8 antibody; PASD 8 antibody; PASD8 antibody; anti-HIF1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HIF1A antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of HIF-1 alpha on mouse small intestine tissue lysate using anti-HIF-1 alpha antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of HIF-1 alpha on mouse small intestine tissue lysate using anti-HIF-1 alpha antibody at 1/500 dilution.)
Related Product Information for anti-HIF1A antibody
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1alpha, HIF-1beta (also designated Arnt 1), EPAS-1 (also designated HIF-2alpha) and HIF-3alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1alpha dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1alpha independent. EPAS-1 and HIF-3alpha have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
Product Categories/Family for anti-HIF1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,670 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
NCBI Protein Information
hypoxia-inducible factor 1-alpha; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; hypoxia-inducible factor1alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MO
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF1A hif1a (Catalog #AAA312108) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF1 alpha Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1 alpha can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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