Application Data
(HLA-G RIIPRHLQL peptide complex tetramer SPRSPR image shows curve for HLA-G tetramer and immobilized Human LILRB2 binding, with a calculated affinity constant of 4.6nM)
HLA-G recombinant protein
HLA-G peptide complex Tetramer
Purity
purity was determined to be >95% by non reducing PAGE and HPLC
Synonyms
HLA-G; N/A; HLA-G peptide complex Tetramer; HLA-G recombinant protein
Host
HEK cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
purity was determined to be >95% by non reducing PAGE and HPLC
Form/Format
Recombinant HLA-G/RIIPRHLQL Tetramer was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding the Human (HLA-G AA25-305 [accession# P17693] and the B2M protein AA2-119 [accession# P61769], fused to the RIIPRHLQL peptide.This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, The tetramer conformation was achieved through biotinylation of the monomer and then covalently binding the monomers via an avidin biotin reaction.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
Application Data
(HLA-G RIIPRHLQL peptide complex tetramer SPRSPR image shows curve for HLA-G tetramer and immobilized Human LILRB2 binding, with a calculated affinity constant of 4.6nM)
Application Data
(HLA-G RIIPRHLQL peptide complex tetramer SPRSPR image shows curve for HLA-G tetramer and immobilized Human LILRB2 binding, with a calculated affinity constant of 4.6nM)
Application Data
(HLA-G RIIPRHLQL peptide complex Tetramer HPLCThe purity of HLA-G RIIPRHLQL peptide complex Tetramer was greater than 95% as determined by SEC-HPLC.)
Application Data
(HLA-G RIIPRHLQL peptide complex Tetramer HPLCThe purity of HLA-G RIIPRHLQL peptide complex Tetramer was greater than 95% as determined by SEC-HPLC.)
SDS-PAGE
(HLA-G RIIPRHLQL peptide complex Tetramer PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
SDS-PAGE
(HLA-G RIIPRHLQL peptide complex Tetramer PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Product Categories/Family for HLA-G recombinant protein
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Product Notes
The HLA-G (Catalog #AAA76365) is a Recombinant Protein produced from HEK cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HLA-G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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