Standard Curve (Sample)
Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) ELISA Kit | HSPA5 elisa kit
Human immunoglobulin binding protein, BIP ELISA Kit
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human
Synonyms
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); N/A; Human immunoglobulin binding protein, BIP ELISA Kit; Human immunoglobulin binding protein; BIP ELISA KIT; BIP; FLJ26106; GRP78; MIF2; Heat-shock 70kD protein-5 (glucose-regulated protein; 78kD) ; heat shock 70kD protein 5 (glucose-regulated protein; heat shock 70kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa); HSPA5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human HSPA5. No significant cross-reactivity or interference between Human HSPA5 and analogues was observed.
Sequence Length
654
Samples
Serum, tissue homogenates
Assay Type
Sandwich
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HSPA5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,333 Da
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
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Product Notes
The Human HSPA5 hspa5 (Catalog #AAA79961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79961 ELISA Kit recognizes Human HSPA5. It is sometimes possible for the material contained within the vial of "heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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