IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-CD73 AD2 Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of AD2 (AAA72309) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72309, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human CD73 Antibody | anti-CD73 antibody
Anti-CD73 [AD2]
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified. Protein A affinity purified
Synonyms
CD73; N/A; Anti-CD73 [AD2]; NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase; anti-CD73 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
AD2
Specificity
This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD73 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-CD73 AD2 Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of AD2 (AAA72309) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72309, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-CD73 AD2 Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of AD2 (AAA72309) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72309, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD73 antibody AD2 . A431 cells were stained with an isotype control or the rabbit-chimeric version of AD2 (AAA72309, blue line) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488 conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD73 antibody AD2 . A431 cells were stained with an isotype control or the rabbit-chimeric version of AD2 (AAA72309, blue line) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488 conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.)
Related Product Information for anti-CD73 antibody
The original antibody was generated by immunizing mice with full-length CD73 purified from human placenta.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
574
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The CD73 nt5e (Catalog #AAA72309) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD73 [AD2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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