IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-Estrogen receptor antibody 4E11 . Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 4E11 (AAA72408) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nucleoli and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72408, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Estrogen receptor Antibody | anti-ESR1 antibody
Anti-Estrogen receptor [4E11]
Gene Names
ESR1; ER; ESR; Era; ESRA; NR3A1
Reactivity
Human
Applications
ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Estrogen receptor; N/A; Anti-Estrogen receptor [4E11]; ER; ERalpha36; ER-alpha36; hER-alpha36; ESR1; ER-alpha; NR3A1; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ER-alpha Estradiol receptor; anti-ESR1 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
4E11
Specificity
The antibody is specific for estrogen receptor ER-alpha36. The estrogen receptor ER-alpha36 is an estrogen receptor subtype mainly expressed on the cell membrane; it mediates the non-genomic estrogen signal pathway, and can inhibit the traditional estrogen signal pathway. It plays a vital role in the occurrence and development of diseases. For example its expression level is closely related to some biological indicators of cancer.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ESR1 antibody
ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-Estrogen receptor antibody 4E11 . Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 4E11 (AAA72408) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nucleoli and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72408, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-Estrogen receptor antibody 4E11 . Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 4E11 (AAA72408) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nucleoli and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72408, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Estrogen receptor antibody 4E11 . Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 4E11 (AAA72408, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Estrogen receptor antibody 4E11 . Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 4E11 (AAA72408, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-ESR1 antibody
The original antibody was generated by immunizing mice with estrogen receptor ER-alpha36.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-ESR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,216 Da
NCBI Official Full Name
estrogen receptor
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
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Product Notes
The ESR1 esr1 (Catalog #AAA72408) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Estrogen receptor [4E11] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen receptor can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen receptor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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