Standard Curve (Sample)
Human A kinase anchor protein 5 (AKAP5) ELISA Kit | AKAP5 elisa kit
Human A kinase anchor protein 5 (AKAP5) ELISA Kit
Gene Names
AKAP5; H21; AKAP75; AKAP79
Reactivity
Human
Synonyms
A kinase anchor protein 5 (AKAP5); N/A; Human A kinase anchor protein 5 (AKAP5) ELISA Kit; AKAP5 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This KAP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KAP5. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AKAP5 elisa kit
Principle of the assay: KAP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KAP5 antibody and an KAP5-HRP conjugate. The assay sample and buffer are incubated together with KAP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KAP5 concentration since KAP5 from samples and KAP5-HRP conjugate compete for the anti-KAP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by KAP5 from the sample, fewer sites are left to bind KAP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KAP5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AKAP5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,088 Da
NCBI Official Full Name
A-kinase anchor protein 5
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 5
NCBI Official Symbol
AKAP5
NCBI Official Synonym Symbols
H21; AKAP75; AKAP79
NCBI Protein Information
A-kinase anchor protein 5; AKAP 79; A-kinase anchor protein 79 kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
UniProt Protein Name
A-kinase anchor protein 5
UniProt Gene Name
AKAP5
UniProt Synonym Gene Names
AKAP79; AKAP-5; AKAP 79
UniProt Entry Name
AKAP5_HUMAN
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Product Notes
The Human AKAP5 akap5 (Catalog #AAA246016) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246016 ELISA Kit recognizes Human AKAP5. It is sometimes possible for the material contained within the vial of "A kinase anchor protein 5 (AKAP5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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