Standard Curve (Sample)
(Fig.1. Human Angiotension II receptor 1 (ANG2R-1) Standard Curve.)
Human Angiotension II Receptor 1 (ANG2R-1) ELISA Kit | ANG2R-1 elisa kit
Human Angiotension II Receptor 1 (ANG2R-1) ELISA Kit
Gene Names
AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
Reactivity
Human
Synonyms
Angiotension II Receptor 1 (ANG2R-1); N/A; Human Angiotension II Receptor 1 (ANG2R-1) ELISA Kit; AGTR1; AG2S; AGTR1A; AGTR1B; AT1; AT1B; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; angiotensin receptor 1; angiotensin receptor 1B; type-1B angiotensin receptor; ANG2R-1 elisa kit
Reactivity
Human
Specificity
Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGTR1. No significant cross-reactivity or interference between Human AGTR1 and analogues was observed.
Sequence Length
359
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Angiotension II receptor 1 (ANG2R-1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Angiotension II receptor 1 (ANG2R-1) Standard Curve.)
Related Product Information for ANG2R-1 elisa kit
Description: This Human Angiotension II receptor 1 (ANG2R-1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGTR1 in samples. An antibody specific for AGTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor. The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.
Background: Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor. The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
type-1 angiotensin II receptor isoform 1
NCBI Official Synonym Full Names
angiotensin II receptor type 1
NCBI Official Symbol
AGTR1
NCBI Official Synonym Symbols
AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
NCBI Protein Information
type-1 angiotensin II receptor
UniProt Protein Name
Type-1 angiotensin II receptor
UniProt Gene Name
AGTR1
UniProt Synonym Gene Names
AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1
UniProt Entry Name
AGTR1_HUMAN
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Product Notes
The Human ANG2R-1 agtr1 (Catalog #AAA331154) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331154 ELISA Kit recognizes Human ANG2R-1. It is sometimes possible for the material contained within the vial of "Angiotension II Receptor 1 (ANG2R-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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