Standard Curve (Sample)
(Fig.1. Human Antigen peptide transporter 1 (TAP1) Standard Curve.)
Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit | TAP1 elisa kit
Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit
Gene Names
TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
Reactivity
Human
Synonyms
Antigen Peptide Transporter 1 (TAP1); N/A; Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit; TAP1; DAAP-57C1.5; ABC17; ABCB2; APT1; D6S114E; FLJ26666; FLJ41500; PSF1; RING4; TAP1*0102N; TAP1N; ABC transporter; MHC 1; ATP-binding cassette; sub-family B (MDR/TAP); member 2; antigen peptide transporte; TAP1 elisa kit
Reactivity
Human
Specificity
Human Antigen peptide transporter 1 (TAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAP1. No significant cross-reactivity or interference between Human TAP1 and analogues was observed.
Sequence Length
808
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Antigen peptide transporter 1 (TAP1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Antigen peptide transporter 1 (TAP1) Standard Curve.)
Related Product Information for TAP1 elisa kit
Description: This Human Antigen peptide transporter 1 (TAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP1 in samples. An antibody specific for TAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The membrane-associated protein encoded by TAP1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Antigen peptide transporter 1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. Antigen peptide transporter 1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in TAP1 may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for TAP1.
Background: The membrane-associated protein encoded by TAP1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Antigen peptide transporter 1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. Antigen peptide transporter 1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in TAP1 may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for TAP1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
antigen peptide transporter 1 isoform 1
NCBI Official Synonym Full Names
transporter 1, ATP binding cassette subfamily B member
NCBI Official Symbol
TAP1
NCBI Official Synonym Symbols
APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
NCBI Protein Information
antigen peptide transporter 1
UniProt Protein Name
Antigen peptide transporter 1
UniProt Gene Name
TAP1
UniProt Synonym Gene Names
ABCB2; PSF1; RING4; Y3; APT1; PSF-1
UniProt Entry Name
TAP1_HUMAN
Similar Products
Product Notes
The Human TAP1 tap1 (Catalog #AAA330685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330685 ELISA Kit recognizes Human TAP1. It is sometimes possible for the material contained within the vial of "Antigen Peptide Transporter 1 (TAP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.