Human ATPase family AAA domain containing protein 3B (ATAD3B) ELISA Kit | ATAD3B elisa kit

Human ATPase family AAA domain containing protein 3B (ATAD3B) ELISA Kit

Cat. Num. AAA27295

• Gene Names: ATAD3B; TOB3; AAA-TOB3
• Reactivity: Human
• Synonyms: ATPase family AAA domain containing protein 3B (ATAD3B); N/A; Human ATPase family AAA domain containing protein 3B (ATAD3B) ELISA Kit; ATAD3B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATAD3B. No significant cross-reactivity or interference between ATAD3B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATAD3B and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ATAD3B elisa kit

Principle of the Assay: ATAD3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATAD3B antibody and an ATAD3B-HRP conjugate. The assay sample and buffer are incubated together with ATAD3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAD3B concentration since ATAD3B from samples and ATAD3B-HRP conjugate compete for the anti-ATAD3B antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAD3B from the sample, fewer sites are left to bind ATAD3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATAD3B concentration in each sample is interpolated from this standard curve.

Intended Uses: This ATAD3B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATAD3B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 75677353
• NCBI GeneID: 83858
• NCBI Accession #: NP_114127.3
• NCBI GenBank Nucleotide #: NM_031921.4
• Molecular Weight: 67,608 Da
• NCBI Official Full Name: ATPase family AAA domain-containing protein 3B
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 3B
• NCBI Official Symbol: ATAD3B
• NCBI Official Synonym Symbols: TOB3; AAA-TOB3
• NCBI Protein Information: ATPase family AAA domain-containing protein 3B; AAA-ATPase TOB3
• UniProt Protein Name: ATPase family AAA domain-containing protein 3B
• UniProt Gene Name: ATAD3B
• UniProt Synonym Gene Names: KIAA1273; TOB3
• UniProt Entry Name: ATD3B_HUMAN