Standard Curve (Sample)
Human ATPase family AAA domain containing protein 3C (ATAD3C) ELISA Kit | ATAD3C elisa kit
Human ATPase family AAA domain containing protein 3C (ATAD3C) ELISA Kit
Reactivity
Human
Synonyms
ATPase family AAA domain containing protein 3C (ATAD3C); N/A; Human ATPase family AAA domain containing protein 3C (ATAD3C) ELISA Kit; ATAD3C elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ATAD3C elisa kit
Intended Uses: This ATAD3C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATAD3C. This ELISA kit for research use only!
Principle of the Assay: ATAD3C ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATAD3C antibody and an ATAD3C-HRP conjugate. The assay sample and buffer are incubated together with ATAD3C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAD3C concentration since ATAD3C from samples and ATAD3C-HRP conjugate compete for the anti-ATAD3C antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAD3C from the sample, fewer sites are left to bind ATAD3C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATAD3C concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ATAD3C ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATAD3C antibody and an ATAD3C-HRP conjugate. The assay sample and buffer are incubated together with ATAD3C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAD3C concentration since ATAD3C from samples and ATAD3C-HRP conjugate compete for the anti-ATAD3C antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAD3C from the sample, fewer sites are left to bind ATAD3C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATAD3C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,380 Da
NCBI Official Full Name
ATPase family AAA domain-containing protein 3C
NCBI Official Synonym Full Names
ATPase family, AAA domain containing 3C
NCBI Official Symbol
ATAD3C
NCBI Protein Information
ATPase family AAA domain-containing protein 3C; ATPase family, AAA domain containing 3A
UniProt Protein Name
ATPase family AAA domain-containing protein 3C
UniProt Gene Name
ATAD3C
UniProt Entry Name
ATD3C_HUMAN
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Product Notes
The Human ATAD3C atad3c (Catalog #AAA246290) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246290 ELISA Kit recognizes Human ATAD3C. It is sometimes possible for the material contained within the vial of "ATPase family AAA domain containing protein 3C (ATAD3C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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