Standard Curve (Sample)
(Human TNFSF13B PicoKine ELISA Kit standard curve)
Human BAFF/TNFSF13B ELISA Kit | TNFSF13B elisa kit
Human BAFF/TNFSF13B PicoKine Quick ELISA Kit
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human
Applications
ELISA
Synonyms
BAFF/TNFSF13B; N/A; Human BAFF/TNFSF13B PicoKine Quick ELISA Kit; TNFSF13B elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFSF13B
Assay Type
Sandwich
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<2 pg/ml
Applicable Applications for TNFSF13B elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human TNFSF13B PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human TNFSF13B PicoKine ELISA Kit standard curve)
Related Product Information for TNFSF13B elisa kit
Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFFR). 3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.
Principle of the Assay: The Quick Picokine™ Human TNFSF13B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human TNFSF13B protein as standards. To measure Human TNFSF13B, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human TNFSF13B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFSF13B in the sample.
Principle of the Assay: The Quick Picokine™ Human TNFSF13B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human TNFSF13B protein as standards. To measure Human TNFSF13B, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human TNFSF13B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFSF13B in the sample.
Product Categories/Family for TNFSF13B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,578 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13B isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol
TNFSF13B
NCBI Official Synonym Symbols
DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13B; ApoL related ligand TALL-1; B-cell-activating factor; B-lymphocyte stimulator; Delta4 BAFF; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; delta BAFF; dendritic
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13B
UniProt Gene Name
TNFSF13B
UniProt Synonym Gene Names
BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
UniProt Entry Name
TN13B_HUMAN
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Product Notes
The Human TNFSF13B tnfsf13b (Catalog #AAA126793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126793 ELISA Kit recognizes Human TNFSF13B. AAA Biotech's BAFF/TNFSF13B can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TNFSF13B tnfsf13b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAFF/TNFSF13B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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