Standard Curve (Sample)
Human BAFF ELISA Kit | TNFSF13B elisa kit
Human BAFF PicoKine ELISA Kit
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human
Synonyms
BAFF; N/A; Human BAFF PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor (ligand) superfamily, member 13b; B lymphocyte stimulator; B-cell-activating factor; BLyS; CD257; Dendritic cell-derived TNF-like molecule; soluble form; TALL-1; TN13B_HUMAN; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF13B; Tumor necrosis factor ligand superfamily member 13b; TNFSF13B elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFSF13B
Sequence Length
285
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<2 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNFSF13B elisa kit
Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.
Principle of the Assay: The Picokine™ Human TNFSF13B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFSF13B with a 96-well strip plate that is pre-coated with antibody specific for TNFSF13B. The detection antibody is a biotinylated antibody specific for TNFSF13B. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFSF13B with immunogen: Expression system for standard: NS0; Immunogen sequence: A134-L285. The kit is analytically validated with ready-to-use reagents. To measure Human TNFSF13B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFSF13B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFSF13B in the sample.
Principle of the Assay: The Picokine™ Human TNFSF13B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFSF13B with a 96-well strip plate that is pre-coated with antibody specific for TNFSF13B. The detection antibody is a biotinylated antibody specific for TNFSF13B. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFSF13B with immunogen: Expression system for standard: NS0; Immunogen sequence: A134-L285. The kit is analytically validated with ready-to-use reagents. To measure Human TNFSF13B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFSF13B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFSF13B in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,578 Da
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 13B
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol
TNFSF13B
NCBI Official Synonym Symbols
DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13B
UniProt Gene Name
TNFSF13B
UniProt Synonym Gene Names
BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
UniProt Entry Name
TN13B_HUMAN
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Product Notes
The Human TNFSF13B tnfsf13b (Catalog #AAA45326) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45326 ELISA Kit recognizes Human TNFSF13B. It is sometimes possible for the material contained within the vial of "BAFF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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