Rabbit anti-Human BAFF/BLyS/TNFSF13B Polyclonal Antibody | anti-TNFSF13B antibody

Anti-BAFF/BLyS/TNFSF13B Antibody, Rabbit Polyclonal

Cat. Num. AAA259484

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: BAFF/BLyS/TNFSF13B, Antibody; Anti-BAFF/BLyS/TNFSF13B Antibody, Rabbit Polyclonal; BAFF/BLyS/TNFSF13B Antibody, Rabbit PAb, Antigen Affinity Purified; tumor necrosis factor (ligand) superfamily, member 13b; Anti-BAFF Antibody; Anti-BLYS Antibody; Anti-CD257 Antibody; Anti-DTL Antibody; Anti-TALL-1 Antibody; Anti-TALL1 Antibody; Anti-THANK Antibody; Anti-TNFSF20 Antibody; Anti-TNLG7A Antibody; Anti-UNQ401/PRO738 Antibody; Anti-ZTNF4 Antibody; anti-TNFSF13B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BAFF/BLyS/TNFSF13B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TNFSF13B antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human BAFF/BLyS/TNFSF13B Protein ( Q9Y275-1; Ala134-Leu285). BAFF/BLyS/TNFSF13B specific IgG was purified by Human BAFF/BLyS/TNFSF13B affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Anti-BAFF rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:35 kDa)

IF (Immunofluorescence)

(BAFF was immunoprecipitated using: Lane A:0.5 mg HL60 Whole Cell Lysate 4 uL anti-BAFF rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-BAFF rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size :31 kDa)

Related Product Information for anti-TNFSF13B antibody

B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is a single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors are a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). BLyS is an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

References

Nardell. B. e. al. (2002. . lymphocyt. stimulato. (BLyS). . therapeuti. trichotom. fo. th. treatmen. o. . lymphocyt. diseases. Leu. Lymphoma. 43(7). 1367-73.
Stoh. W. (2006. Therapeuti. targetin. o. . lymphocyt. stimulato. (BLyS. i. th. rheumati. diseases. Endoc. Meta. Immun. Disor. Dru. Targets. 6(4). 51-8.
Cancr. MP. e. al. (2009. Th. rol. o. . lymphocyt. stimulato. (BLyS. i. systemi. lupu. erythematosus. . Cli. Invest. 119(5). 1066-73.

NCBI and Uniprot Product Information

• NCBI GI #: 224548983
• NCBI GeneID: 10673
• NCBI Accession #: NP_001139117.1
• NCBI GenBank Nucleotide #: NM_001145645.2
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• Molecular Weight: 17,578 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B; ApoL related ligand TALL-1; B-cell-activating factor; B-lymphocyte stimulator; Delta4 BAFF; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; delta BAFF; dendritic
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN