Standard Curve (Sample)
Human Caspase 3 (CASP3) ELISA Kit | CASP3 elisa kit
Human Caspase 3 (CASP3) ELISA Kit
Reactivity
Human
Synonyms
Caspase 3 (CASP3); N/A; Human Caspase 3 (CASP3) ELISA Kit; CASP3 elisa kit
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural human CASP3. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity. No significant cross-reactivity was observed.
Assay Type
Quantitative Sandwich
Sensitivity
< 0.031ng / mL
Intra-assay Precision
Intra-plate Precision 3 samples with low, middle and high level CASP3 were tested 16 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Intra-plate Precision 3 samples with low, middle and high level CASP3 were tested 16 times on one plate, respectively. Intra-Assay: CV<10%
Preparation and Storage
Store unopened kit at 2-8 degree C for1 week. If more than one week, please keep the components of the kit according to the instructions.Do not use past kit expiration date.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CASP3 elisa kit
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in cell lysates, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human CASP3 has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any CASP3 present is bound by the immobilized antibody. After washing away any unbound substances, and then a detection antibody specific for CASP3 is added to the wells and binds to the combination of capture antibody CASP3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount ofCASP3 bound in the initial step. The color development is stopped and the absorbance is measured.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human CASP3 has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any CASP3 present is bound by the immobilized antibody. After washing away any unbound substances, and then a detection antibody specific for CASP3 is added to the wells and binds to the combination of capture antibody CASP3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount ofCASP3 bound in the initial step. The color development is stopped and the absorbance is measured.
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Product Notes
The Human CASP3 (Catalog #AAA282156) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282156 ELISA Kit recognizes Human CASP3. It is sometimes possible for the material contained within the vial of "Caspase 3 (CASP3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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