Standard Curve (Sample)
Human CD105 ELISA Kit | CD105 elisa kit
Human CD105 PicoKine Fast ELISA Kit
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Human
Synonyms
CD105; N/A; Human CD105 PicoKine Fast ELISA Kit; Human CD105; Endoglin; ENG; END; CD105 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human ENG
Sequence Length
625
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD105 elisa kit
Principle of the Assay: The Picokine™ Human ENG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ENG with a 96-well strip plate that is pre-coated with antibody specific for ENG. The detection antibody is a biotinylated antibody specific for ENG. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ENG with immunogen: Expression system for standard: CHO; Immunogen sequence: E26-G586. The kit is analytically validated with ready to use reagents. To measure Human ENG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human ENG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ENG in the sample.!!Background/Introduction: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
endoglin isoform 2
NCBI Official Synonym Full Names
endoglin
NCBI Official Symbol
ENG
NCBI Official Synonym Symbols
END; HHT1; ORW1
NCBI Protein Information
endoglin
UniProt Protein Name
Endoglin
UniProt Gene Name
ENG
UniProt Synonym Gene Names
END
UniProt Entry Name
EGLN_HUMAN
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Product Notes
The Human CD105 eng (Catalog #AAA125339) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125339 ELISA Kit recognizes Human CD105. It is sometimes possible for the material contained within the vial of "CD105, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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