Application Data
Rat anti-Mouse CD105/Endoglin Monoclonal Antibody | anti-CD105 antibody
Rat Anti-Mouse CD105/Endoglin
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein G purified
Synonyms
CD105/Endoglin, Antibody; Rat Anti-Mouse CD105/Endoglin; Anti-mouse CD105/Endoglin-PE (#MJ7/18); Cell surface MJ7/18 antigen; CD105; Endoglin; anti-CD105 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MJ7/18 / IgG2a
Specificity
The unconjugated antibody will detect native mouse CD105 in Western analysis and on the surface of mouse endothelial cells.
Purity/Purification
Protein G purified
Form/Format
Liquid
Concentration
200 ug/ml (varies by lot)
Sequence Length
653
Applicable Applications for anti-CD105 antibody
FCM/FACS (Flow Cytometry)
Immunogen
murine stromal cell line
Conjugation
PE
Preparation
Monoclonal antibodies were produced by immunizing rats with inflamed mouse skin.
Buffer/Stabilizer
PBS; 1% BSA
Preservative
0,02% sodium azide
Warning
Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.
Preparation and Storage
Store protected from light at 2-8 degree C. DO NOT FREEZE!
Application Data
Application Data
Related Product Information for anti-CD105 antibody
Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 70-75 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic teails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondroytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pre-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70% and 97% amino acid sequence identity in their extracellular and intracellular domains, respectively. Endoglin binds TGF-beta1 and TGF-beta3 but not TGF-beta2 efficiently by associating with TGF-beta tyme II receptor (TbetaRII). Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.
References
1. Cheifetz et al., J Biol Chem 267:19027, 1992 2. Parker et al., J Bone Miner Res 18:289, 2003 3. Barbara et al., J Biol Chem 274:584, 1999 4. McAllister et al., Nature Genet 8:345, 1994 5. Chen et al., Proc Natl Acad Sci 99:15468, 2002
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,021 Da
NCBI Official Full Name
endoglin isoform 1
NCBI Official Synonym Full Names
endoglin
NCBI Official Symbol
Eng
NCBI Official Synonym Symbols
Endo; CD105; AI528660; AI662476; S-endoglin
NCBI Protein Information
endoglin; transmembrane glycoprotein; cell surface MJ7/18 antigen
UniProt Protein Name
Endoglin
UniProt Gene Name
Eng
UniProt Synonym Gene Names
Edg
UniProt Entry Name
EGLN_MOUSE
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Product Notes
The CD105 eng (Catalog #AAA79109) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD105/Endoglin reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD105/Endoglin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD105 eng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD105/Endoglin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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