Human CD42b ELISA Kit | CD42b elisa kit

Human CD42b ELISA Kit PicoKine

Cat. Num. AAA127962

• Gene Names: GP1BA; BSS; GP1B; VWDP; CD42B; GPIbA; BDPLT1; BDPLT3; DBPLT3; CD42b-alpha
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD42b; N/A; Human CD42b ELISA Kit PicoKine; CD42b elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: H17-L505
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CD42b elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
  O.D.	0.023	0.096	0.182	0.302	0.595	0.968	1.341	2.043

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	264	1499	5696	259	1432	5590
  Standard deviation	14.52	77.95	205.06	13.21	64.44	268.32
  CV (%)	5.5%	5.2%	3.6%	5.1%	4.5%	4.8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	264	247	219	251	245	9.32	3.8%
  Sample 2	1499	1455	1324	1378	1414	50.9	3.6%
  Sample 3	5696	5411	5439	5282	5457	223.74	4.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human CD42b PicoKine ELISA Kit standard curve)

Related Product Information for CD42b elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CD42b elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 613503880
• NCBI GeneID: 2811
• UniProt Accession #: P07359; Q14441; Q16469; Q8N1F3; Q8NG39; Q9HDC7; Q9UEK1; Q9UQS4; E7ES66
• Molecular Weight: 626
• NCBI Official Full Name: Platelet glycoprotein Ib alpha chain
• NCBI Official Synonym Full Names: glycoprotein Ib (platelet), alpha polypeptide
• NCBI Official Symbol: GP1BA
• NCBI Official Synonym Symbols: BSS; GP1B; VWDP; CD42B; GPIbA; BDPLT1; BDPLT3; DBPLT3; CD42b-alpha
• NCBI Protein Information: platelet glycoprotein Ib alpha chain; GP-Ib alpha; antigen CD42b-alpha; platelet membrane glycoprotein 1b-alpha subunit
• UniProt Protein Name: Platelet glycoprotein Ib alpha chain
• UniProt Gene Name: GP1BA
• UniProt Synonym Gene Names: GP-Ib alpha; GPIb-alpha; GPIbA; Glycoprotein Ibalpha
• UniProt Entry Name: GP1BA_HUMAN