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product-image-AAA263017_SC15.jpg Standard Curve (Sample)

Human CD97 ELISA Kit | CD97 elisa kit

Human CD97 ELISA Kit

Gene Names
ADGRE5; CD97; TM7LN1
Reactivity
Human
Synonyms
CD97; N/A; Human CD97 ELISA Kit; CD97 antigen; Leukocyte antigen CD97; CD97 elisa kit
Ordering
Reactivity
Human
Specificity
The Human CD97 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD97 proteins within the range of 125 pg/ml - 8000 pg/ml.
Sequence Length
786
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

product-image-AAA263017_SC15.jpg Standard Curve (Sample)
Related Product Information for CD97 elisa kit
Principle of the Assay: The Human CD97 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD97 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human CD97 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD97 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD97 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD97 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
976
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,628 Da
NCBI Official Full Name
CD97 antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E5
NCBI Official Symbol
ADGRE5
NCBI Official Synonym Symbols
CD97; TM7LN1
NCBI Protein Information
CD97 antigen
UniProt Protein Name
CD97 antigen
UniProt Gene Name
CD97
UniProt Entry Name
CD97_HUMAN

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Product Notes

The Human CD97 cd97 (Catalog #AAA263017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263017 ELISA Kit recognizes Human CD97. It is sometimes possible for the material contained within the vial of "CD97, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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