Human Cortactin (Src substrate cortactin) ELISA Kit | CTTN elisa kit

Human Cortactin (Src substrate cortactin) ELISA Kit

Cat. Num. AAA250612

• Gene Names: CTTN; EMS1
• Reactivity: Human
• Synonyms: Cortactin (Src substrate cortactin); N/A; Human Cortactin (Src substrate cortactin) ELISA Kit; Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; CTTN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cortactin. No significant crossreactivity or interference between Cortactin and analogues was observed.
• Assay Type: Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Related Product Information for CTTN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96- well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP -Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 296080746
• NCBI GeneID: 2017
• NCBI Accession #: NP_001171669.1
• NCBI GenBank Nucleotide #: NM_001184740.1
• UniProt Accession #: Q14247; Q8N707; Q96H99; AAB26248
• Molecular Weight: 61,586 Da
• NCBI Official Full Name: src substrate cortactin isoform c
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1
• NCBI Protein Information: src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)
• UniProt Protein Name: Src substrate cortactin
• UniProt Gene Name: CTTN
• UniProt Synonym Gene Names: EMS1
• UniProt Entry Name: SRC8_HUMAN