Human Cystine/glutamate transporter (SLC7A11) ELISA Kit | SLC7A11 elisa kit

Human Cystine/glutamate transporter (SLC7A11) ELISA Kit

Cat. Num. AAA27370

• Gene Names: SLC7A11; xCT; CCBR1
• Reactivity: Human
• Synonyms: Cystine/glutamate transporter (SLC7A11); N/A; Human Cystine/glutamate transporter (SLC7A11) ELISA Kit; SLC7A11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC7A11. No significant cross-reactivity or interference between SLC7A11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC7A11 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for SLC7A11 elisa kit

Principle of the Assay: SLC7A11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLC7A11 antibody and an SLC7A11-HRP conjugate. The assay sample and buffer are incubated together with SLC7A11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC7A11 concentration since SLC7A11 from samples and SLC7A11-HRP conjugate compete for the anti-SLC7A11 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC7A11 from the sample, fewer sites are left to bind SLC7A11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC7A11 concentration in each sample is interpolated from this standard curve.

Intended Uses: This SLC7A11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC7A11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 7657683
• NCBI GeneID: 23657
• NCBI Accession #: NP_055146.1
• NCBI GenBank Nucleotide #: NM_014331.3
• Molecular Weight: 55,423 Da
• NCBI Official Full Name: cystine/glutamate transporter
• NCBI Official Synonym Full Names: solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
• NCBI Official Symbol: SLC7A11
• NCBI Official Synonym Symbols: xCT; CCBR1
• NCBI Protein Information: cystine/glutamate transporter; amino acid transport system xc-; solute carrier family 7 member 11; calcium channel blocker resistance protein CCBR1; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• UniProt Protein Name: Cystine/glutamate transporter
• UniProt Gene Name: SLC7A11
• UniProt Entry Name: XCT_HUMAN