Standard Curve (Sample)
(Fig.1. Human Cystine/glutamate transporter (SLC7A11) Standard Curve.)
Human Cystine/Glutamate Transporter (SLC7A11) ELISA Kit | SLC7A11 elisa kit
Human Cystine/Glutamate Transporter (SLC7A11) ELISA Kit
Gene Names
SLC7A11; xCT; CCBR1
Reactivity
Human
Synonyms
Cystine/Glutamate Transporter (SLC7A11); N/A; Human Cystine/Glutamate Transporter (SLC7A11) ELISA Kit; SLC7A11; CCBR1; xCT; cystine/glutamate transporter; SLC7A11 elisa kit
Reactivity
Human
Specificity
Human Cystine/glutamate transporter (SLC7A11) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC7A11. No significant cross-reactivity or interference between Human SLC7A11 and analogues was observed.
Sequence Length
501
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Cystine/glutamate transporter (SLC7A11) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Cystine/glutamate transporter (SLC7A11) Standard Curve.)
Related Product Information for SLC7A11 elisa kit
Description: This Human Cystine/glutamate transporter (SLC7A11) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A11 in samples. An antibody specific for SLC7A11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC7A11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC7A11 is a member of a heteromeric Na(+)-independent anionic amino acid transport system highly specific for cystine and glutamate. In this system, designated system Xc(-), the anionic form of cystine is transported in exchange for glutamate. The deduced 502-amino acid protein had an apparent molecular mass of about 40 kD following in vitro translation in canine pancreatic microsomes. Mouse xCT was found to be extremely hydrophobic and was predicted to contain 12 membrane-spanning domains. Northern blot analysis of activated mouse macrophages detected transcripts of 12, 3.5, and 2.5 kb. Northern blot analysis of mouse tissues detected expression in brain, but not in other organs tested. The 501-amino acid XCT protein has 12 transmembrane domains and cytoplasmic N and C termini.
Background: SLC7A11 is a member of a heteromeric Na(+)-independent anionic amino acid transport system highly specific for cystine and glutamate. In this system, designated system Xc(-), the anionic form of cystine is transported in exchange for glutamate. The deduced 502-amino acid protein had an apparent molecular mass of about 40 kD following in vitro translation in canine pancreatic microsomes. Mouse xCT was found to be extremely hydrophobic and was predicted to contain 12 membrane-spanning domains. Northern blot analysis of activated mouse macrophages detected transcripts of 12, 3.5, and 2.5 kb. Northern blot analysis of mouse tissues detected expression in brain, but not in other organs tested. The 501-amino acid XCT protein has 12 transmembrane domains and cytoplasmic N and C termini.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cystine/glutamate transporter
NCBI Official Synonym Full Names
solute carrier family 7 member 11
NCBI Official Symbol
SLC7A11
NCBI Official Synonym Symbols
xCT; CCBR1
NCBI Protein Information
cystine/glutamate transporter
UniProt Protein Name
Cystine/glutamate transporter
UniProt Gene Name
SLC7A11
UniProt Entry Name
XCT_HUMAN
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Product Notes
The Human SLC7A11 slc7a11 (Catalog #AAA330799) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330799 ELISA Kit recognizes Human SLC7A11. It is sometimes possible for the material contained within the vial of "Cystine/Glutamate Transporter (SLC7A11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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