Standard Curve (Sample)
Human Endolyn (CD164) ELISA Kit | CD164 elisa kit
Human Endolyn (CD164) ELISA Kit
Gene Names
CD164; DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
Reactivity
Human
Synonyms
Endolyn (CD164); N/A; Human Endolyn (CD164) ELISA Kit; CD164 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CD164
Detection Range
93.8 pg/ml - 6,000 pg/ml
Sensitivity
<50 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter- assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD164 elisa kit
Background: Sialomucin core protein 24 also known as endolyn or CD164, is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
Principle of the Assay: To measure Human CD164, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CD164 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD164 in the sample.
Intended Uses: This ELISA kit is a solid-phase immunoassay specially designed to measure Human CD164 with a 96-well strip plate that is pre-coated with antibody specific for CD164. The detection antibody is a biotinylated antibody specific for CD164. The capture antibody is A monoclonal antibody from mouse and the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit includes Human CD164 protein as standards.
Principle of the Assay: To measure Human CD164, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CD164 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD164 in the sample.
Intended Uses: This ELISA kit is a solid-phase immunoassay specially designed to measure Human CD164 with a 96-well strip plate that is pre-coated with antibody specific for CD164. The detection antibody is a biotinylated antibody specific for CD164. The capture antibody is A monoclonal antibody from mouse and the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit includes Human CD164 protein as standards.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,736 Da
NCBI Official Full Name
Sialomucin core protein 24
NCBI Official Synonym Full Names
CD164 molecule
NCBI Official Symbol
CD164
NCBI Official Synonym Symbols
DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
NCBI Protein Information
sialomucin core protein 24
UniProt Protein Name
Sialomucin core protein 24
UniProt Gene Name
CD164
UniProt Synonym Gene Names
MUC-24; MGC-24; MGC-24v
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Product Notes
The Human CD164 cd164 (Catalog #AAA37889) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37889 ELISA Kit recognizes Human CD164. It is sometimes possible for the material contained within the vial of "Endolyn (CD164), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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