Standard Curve (Sample)
Human CD164 ELISA Kit | CD164 elisa kit
Human CD164 PicoKine ELISA Kit
Gene Names
CD164; DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
Reactivity
Human
Synonyms
CD164; N/A; Human CD164 PicoKine ELISA Kit; Human CD164; CD164 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CD14
Sequence Length
197
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD164 elisa kit
Principle of the Assay: The Picokine Human CD14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD14 with a 96-well strip plate that is pre-coated with antibody specific for CD14. The detection antibody is a biotinylated antibody specific for CD14. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CD14 with immunogen: Expression system for standard: CHO, Immunogen sequence: T20-C352. The kit is analytically validated with ready to use reagents. To measure Human CD14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD14 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD14 in the sample!!Background/Introduction: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50-to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Human CD164 (Catalog #AAA125055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125055 ELISA Kit recognizes Human CD164. It is sometimes possible for the material contained within the vial of "CD164, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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