FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD164 expression on human whole blood monocytes. Cells were stained with purified anti-Human CD164, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
Mouse anti-Human CD164 Monoclonal Antibody | anti-CD164 antibody
Anti-CD164 Antibody, Mouse Monoclonal
Gene Names
CD164; DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Protein A
Synonyms
CD164, Antibody; Anti-CD164 Antibody, Mouse Monoclonal; CD164 Antibody, Mouse MAb; CD164 molecule, sialomucin; Anti-endolyn Antibody; Anti-MGC-24 Antibody; Anti-MUC-24 Antibody; anti-CD164 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
05
Specificity
Human CD164
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD164 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human CD164 Protein
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD164 (rh CD164). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD164 expression on human whole blood monocytes. Cells were stained with purified anti-Human CD164, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD164 expression on human whole blood monocytes. Cells were stained with purified anti-Human CD164, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CD164 in human pancreatic carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections))
IHC (Immunohiostchemistry)
(Immunochemical staining of human CD164 in human pancreatic carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections))
IHC (Immunohistochemistry)
(Immunochemical staining of human CD164 in human prostatic carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD164 in human prostatic carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)
References
McGuckin CP, et al. (2003) Colocalization analysis of sialomucins CD34 and CD164. Stem Cells. 21(2): 162-70.Doyonnas R, et al. (2000) CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 165(2): 840-51.Zannettino AC, et al. (1998) The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 92(8): 2613-28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Sialomucin core protein 24
NCBI Official Synonym Full Names
CD164 molecule
NCBI Official Symbol
CD164
NCBI Official Synonym Symbols
DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
NCBI Protein Information
sialomucin core protein 24
UniProt Protein Name
Sialomucin core protein 24
UniProt Gene Name
CD164
UniProt Synonym Gene Names
MUC-24; MGC-24; MGC-24v
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Product Notes
The CD164 cd164 (Catalog #AAA255552) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD164 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD164 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CD164 cd164 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD164, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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