Human IL-6 ELISA Kit | IL-6 elisa kit

Human IL-6 ELISA KIT

Cat. Num. AAA309860

• Gene Names: Il6; Il-6
• Reactivity: Human
• Synonyms: IL-6; N/A; Human IL-6 ELISA KIT; Human IL-6; IL-6 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL-6 Ligand
• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 3.9-250pg/mL
• Sensitivity: 2pg/mL

• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Standard Curve (Sample)

(Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for IL-6 elisa kit

Interleukin 6 (IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa (6-9). The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence (10). On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene (11-13).The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion (6, 14 - 17). IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-g (18-20). IL-6 stimulates production of acute phase proteins by hepatocytes (21) and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells (4, 5).

NCBI and Uniprot Product Information

• NCBI GI #: 124348
• NCBI GeneID: 16193
• UniProt Accession #: P08505; Q3UCQ0; Q8BN26
• Molecular Weight: 24,384 Da
• NCBI Official Full Name: Interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: Il-6
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6