Standard Curve (Sample)
(Fig.1. Human Low-density lipoprotein-receptor-related protein (LRP-6) Standard Curve.)
Human Low-Density Lipoprotein-receptor-Related Protein (LRP-6) ELISA Kit | LRP-6 elisa kit
Human Low-Density Lipoprotein-receptor-Related Protein (LRP-6) ELISA Kit
Gene Names
LRP6; ADCAD2; STHAG7
Reactivity
Human
Synonyms
Low-Density Lipoprotein-receptor-Related Protein (LRP-6); N/A; Human Low-Density Lipoprotein-receptor-Related Protein (LRP-6) ELISA Kit; LRP6; ADCAD2; FLJ90062; FLJ90421; LRP-6 elisa kit
Reactivity
Human
Specificity
Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRP6. No significant cross-reactivity or interference between Human LRP6 and analogues was observed.
Sequence Length
1613
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Low-density lipoprotein-receptor-related protein (LRP-6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Low-density lipoprotein-receptor-related protein (LRP-6) Standard Curve.)
Related Product Information for LRP-6 elisa kit
Description: This Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP6 in samples. An antibody specific for LRP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The LRP6 is a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.
Background: The LRP6 is a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
low-density lipoprotein receptor-related protein 6
NCBI Official Synonym Full Names
LDL receptor related protein 6
NCBI Official Symbol
LRP6
NCBI Official Synonym Symbols
ADCAD2; STHAG7
NCBI Protein Information
low-density lipoprotein receptor-related protein 6
UniProt Protein Name
Low-density lipoprotein receptor-related protein 6
UniProt Gene Name
LRP6
UniProt Synonym Gene Names
LRP-6
UniProt Entry Name
LRP6_HUMAN
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Product Notes
The Human LRP-6 lrp6 (Catalog #AAA331927) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331927 ELISA Kit recognizes Human LRP-6. It is sometimes possible for the material contained within the vial of "Low-Density Lipoprotein-receptor-Related Protein (LRP-6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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