Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) Standard Curve.)
Human Mediator Of RNA Polymerase II Transcription Subunit 13-Like (MED13L) ELISA Kit | MED13L elisa kit
Human Mediator Of RNA Polymerase II Transcription Subunit 13-Like (MED13L) ELISA Kit
Gene Names
MED13L; MRFACD; THRAP2; TRAP240L; PROSIT240
Reactivity
Human
Synonyms
Mediator Of RNA Polymerase II Transcription Subunit 13-Like (MED13L); N/A; Human Mediator Of RNA Polymerase II Transcription Subunit 13-Like (MED13L) ELISA Kit; MED13L; DKFZp781D0112; FLJ21627; KIAA1025; PROSIT240; THRAP2; TRAP240L; thyroid hormone receptor associated protein 2; MED13L elisa kit
Reactivity
Human
Specificity
Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED13L. No significant cross-reactivity or interference between Human MED13L and analogues was observed.
Sequence Length
2210
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) Standard Curve.)
Related Product Information for MED13L elisa kit
Description: This Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13L in samples. An antibody specific for MED13L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MED13L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mediator of RNA polymerase II transcription subunit 13-like encoded by MED13L is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. Mediator of RNA polymerase II transcription subunit 13-like is involved in early development of the heart and brain. Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA).
Background: Mediator of RNA polymerase II transcription subunit 13-like encoded by MED13L is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. Mediator of RNA polymerase II transcription subunit 13-like is involved in early development of the heart and brain. Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 13-like
NCBI Official Synonym Full Names
mediator complex subunit 13L
NCBI Official Symbol
MED13L
NCBI Official Synonym Symbols
MRFACD; THRAP2; TRAP240L; PROSIT240
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 13-like
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 13-like
UniProt Gene Name
MED13L
UniProt Synonym Gene Names
KIAA1025; PROSIT240; THRAP2; TRAP240L
UniProt Entry Name
MD13L_HUMAN
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Product Notes
The Human MED13L med13l (Catalog #AAA331818) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331818 ELISA Kit recognizes Human MED13L. It is sometimes possible for the material contained within the vial of "Mediator Of RNA Polymerase II Transcription Subunit 13-Like (MED13L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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