WB (Western Blot)
(Detection of human MED13L by western blot. Samples: Whole cell lysate (15 ug) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody AAA211867 (lot AAA211867-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MED13L Polyclonal Antibody | anti-MED13L antibody
Rabbit anti-MED13L Antibody, Affinity Purified
Gene Names
MED13L; THRAP2; TRAP240L; PROSIT240
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MED13L, Antibody; Rabbit anti-MED13L Antibody, Affinity Purified; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like; THRAP2; PROSIT240; MRFACD; mediator of RNA polymerase II transcription subunit 13-like; Mediator complex subunit 13-like; TRAP240L; Mediator of RNA polymerase II transcription subunit 13-like; mediator complex subunit 13 like; anti-MED13L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MED13L antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 550 and 600
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MED13L by western blot. Samples: Whole cell lysate (15 ug) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody AAA211867 (lot AAA211867-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human MED13L by western blot. Samples: Whole cell lysate (15 ug) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody AAA211867 (lot AAA211867-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MED13L antibody AAA211867 (lot AAA211867-3) used for IP at 6 ug per reaction. MED13L was also immunoprecipitated by a previous lot of this antibody (lot AAA211867-2) and rabbit anti-MED13L antibody For blotting immunoprecipitated MED13L, AAA211867 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MED13L antibody AAA211867 (lot AAA211867-3) used for IP at 6 ug per reaction. MED13L was also immunoprecipitated by a previous lot of this antibody (lot AAA211867-2) and rabbit anti-MED13L antibody For blotting immunoprecipitated MED13L, AAA211867 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human MED13L by immunohistochemistry. Sample:FFPE section of human lung carcinoma. Antibody:Affinity purified rabbit anti-MED13L (Cat. No. AAA211867 lot 3) used at 1:1,000 (0.2ug/ml).Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MED13L by immunohistochemistry. Sample:FFPE section of human lung carcinoma. Antibody:Affinity purified rabbit anti-MED13L (Cat. No. AAA211867 lot 3) used at 1:1,000 (0.2ug/ml).Detection: DAB)
Related Product Information for anti-MED13L antibody
Mediator complex subunit 13-like is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q71F56].
Product Categories/Family for anti-MED13L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
242,602 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 13-like
NCBI Official Synonym Full Names
mediator complex subunit 13-like
NCBI Official Symbol
MED13L
NCBI Official Synonym Symbols
THRAP2; TRAP240L; PROSIT240
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 13-like; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 13-like
UniProt Gene Name
MED13L
UniProt Synonym Gene Names
KIAA1025; PROSIT240; THRAP2; TRAP240L
UniProt Entry Name
MD13L_HUMAN
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Product Notes
The MED13L med13l (Catalog #AAA211867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MED13L Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED13L can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MED13L med13l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED13L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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