Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 14 (MED14) Standard Curve.)
Human Mediator Of RNA Polymerase II Transcription Subunit 14 (MED14) ELISA Kit | MED14 elisa kit
Human Mediator Of RNA Polymerase II Transcription Subunit 14 (MED14) ELISA Kit
Gene Names
MED14; CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
Reactivity
Human
Synonyms
Mediator Of RNA Polymerase II Transcription Subunit 14 (MED14); N/A; Human Mediator Of RNA Polymerase II Transcription Subunit 14 (MED14) ELISA Kit; MED14; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; MGC104513; RGR1; TRAP170; cofactor required for Sp1 transcriptional activation; subunit 2 (150kD); MED14 elisa kit
Reactivity
Human
Specificity
Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED14. No significant cross-reactivity or interference between Human MED14 and analogues was observed.
Sequence Length
1454
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 14 (MED14) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mediator of RNA polymerase II transcription subunit 14 (MED14) Standard Curve.)
Related Product Information for MED14 elisa kit
Description: This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 14 encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Mediator of RNA polymerase II transcription subunit 14 contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.
Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 14 encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Mediator of RNA polymerase II transcription subunit 14 contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 14
NCBI Official Synonym Full Names
mediator complex subunit 14
NCBI Official Symbol
MED14
NCBI Official Synonym Symbols
CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 14
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 14
UniProt Gene Name
MED14
UniProt Synonym Gene Names
ARC150; CRSP2; CXorf4; DRIP150; EXLM1; RGR1; TRAP170; ARC150; CRSP complex subunit 2; hRGR1; Trap170; DRIP150
UniProt Entry Name
MED14_HUMAN
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Product Notes
The Human MED14 med14 (Catalog #AAA331817) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331817 ELISA Kit recognizes Human MED14. It is sometimes possible for the material contained within the vial of "Mediator Of RNA Polymerase II Transcription Subunit 14 (MED14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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