Human Metalloreductase STEAP1 (STEAP1) ELISA Kit | STEAP1 elisa kit

Human Metalloreductase STEAP1 (STEAP1) ELISA Kit

Cat. Num. AAA246284

• Gene Names: STEAP1; STEAP; PRSS24
• Reactivity: Human
• Synonyms: Metalloreductase STEAP1 (STEAP1); N/A; Human Metalloreductase STEAP1 (STEAP1) ELISA Kit; STEAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of STEAP1. No significant cross-reactivity or interference between STEAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STEAP1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for STEAP1 elisa kit

Principle of the Assay: STEAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-STEAP1 antibody and an STEAP1-HRP conjugate. The assay sample and buffer are incubated together with STEAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STEAP1 concentration since STEAP1 from samples and STEAP1-HRP conjugate compete for the anti-STEAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by STEAP1 from the sample, fewer sites are left to bind STEAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STEAP1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This STEAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STEAP1. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for STEAP1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 9558759
• NCBI GeneID: 26872
• NCBI Accession #: NP_036581.1
• NCBI GenBank Nucleotide #: NM_012449.2
• Molecular Weight: 39,851 Da
• NCBI Official Full Name: metalloreductase STEAP1
• NCBI Official Synonym Full Names: six transmembrane epithelial antigen of the prostate 1
• NCBI Official Symbol: STEAP1
• NCBI Official Synonym Symbols: STEAP; PRSS24
• NCBI Protein Information: metalloreductase STEAP1; six-transmembrane epithelial antigen of prostate 1
• UniProt Protein Name: Metalloreductase STEAP1
• UniProt Gene Name: STEAP1
• UniProt Synonym Gene Names: PRSS24; STEAP
• UniProt Entry Name: STEA1_HUMAN