WB (Western Blot)
(The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: U87MG cell lysate;;)
Mouse anti-Human STEAP1 Monoclonal Antibody | anti-STEAP1 antibody
STEAP1
Gene Names
STEAP1; STEAP; PRSS24
Reactivity
Human
Applications
Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
STEAP1, Antibody; STEAP1; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; PRSS24; anti-STEAP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
J2D2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
486
Applicable Applications for anti-STEAP1 antibody
WB (Western Blot), ELISA
Immunogen
Recombinant human STEAP1 (1-70aa) purified from E. coli
Conjugation
Unconjugated
Product Note
This product was produced from tissue culture supernatant.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: U87MG cell lysate;;)
WB (Western Blot)
(The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: U87MG cell lysate;;)
Related Product Information for anti-STEAP1 antibody
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostatetissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing'ssarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysisindicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostatecancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested itspotential use as a target for immunotherapy.
Product Categories/Family for anti-STEAP1 antibody
References
David A. Rodeberg, et al. (2005) Clin Cancer Res. June11(12): 4545-4552.;;Pia M. Challita-Eid, et al., (2007) Cancer research June 67(12): 5798-5805.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
similar to Six transmembrane epithelial antigen of prostate
NCBI Official Synonym Full Names
STEAP family member 1
NCBI Official Symbol
STEAP1
NCBI Official Synonym Symbols
STEAP; PRSS24
NCBI Protein Information
metalloreductase STEAP1
UniProt Protein Name
Metalloreductase STEAP1
UniProt Gene Name
STEAP1
UniProt Synonym Gene Names
PRSS24; STEAP
UniProt Entry Name
STEA1_HUMAN
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Product Notes
The STEAP1 steap1 (Catalog #AAA48301) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STEAP1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STEAP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the STEAP1 steap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STEAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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