Standard Curve (Sample)
Human Mothers Against Decapentaplegic Homolog 9 (SMAD9) ELISA Kit | SMAD9 elisa kit
Human Mothers Against Decapentaplegic Homolog 9 (SMAD9) ELISA Kit
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
Reactivity
Human
Synonyms
Mothers Against Decapentaplegic Homolog 9 (SMAD9); N/A; Human Mothers Against Decapentaplegic Homolog 9 (SMAD9) ELISA Kit; SMAD9; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; MAD (mothers against decapentaplegic; Drosophila) homolog 9; MAD; mothers against decapentaplegic homolog 9; Mothers against decapentaplegic; drosophila; homolog o; SMAD9 elisa kit
Reactivity
Human
Specificity
Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD9. No significant cross-reactivity or interference between Human SMAD9 and analogues was observed.
Sequence Length
467
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
0.25 ng/ml - 4 ng/ml
Sensitivity
< 0.01ng/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SMAD9 elisa kit
Background: SMAD9 or Mothers against decapentaplegic homolog 9 is a polypeptide that, as its namedescribes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFbeta family members, SMAD9 is involved in cell signalling.When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).
Principle of the Assay: Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9 in samples. An antibody specific for SMAD9 has been pre-coated onto a microplate. Standards and samples are pipetted intothe wells and any SMAD9 present is bound by the immobilized antibody. After removingany unbound substances, HRP-Conjugated Human SMAD9 detection antibody is addedto the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SMAD9bound in the initial step. The color development is stopped and the intensity of the color ismeasured.
Principle of the Assay: Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9 in samples. An antibody specific for SMAD9 has been pre-coated onto a microplate. Standards and samples are pipetted intothe wells and any SMAD9 present is bound by the immobilized antibody. After removingany unbound substances, HRP-Conjugated Human SMAD9 detection antibody is addedto the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SMAD9bound in the initial step. The color development is stopped and the intensity of the color ismeasured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mothers against decapentaplegic homolog 9
NCBI Official Synonym Full Names
SMAD family member 9
NCBI Official Symbol
SMAD9
NCBI Official Synonym Symbols
PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
NCBI Protein Information
mothers against decapentaplegic homolog 9
UniProt Protein Name
Mothers against decapentaplegic homolog 9
UniProt Gene Name
SMAD9
UniProt Synonym Gene Names
MADH6; MADH9; MAD homolog 9; Mothers against DPP homolog 9; SMAD 9; Smad9
UniProt Entry Name
SMAD9_HUMAN
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Product Notes
The Human SMAD9 smad9 (Catalog #AAA330782) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330782 ELISA Kit recognizes Human SMAD9. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 9 (SMAD9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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