IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.)
Rabbit anti-Human SMAD9 Polyclonal Antibody | anti-SMAD9 antibody
SMAD9 Antibody (Center)
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMAD9, Antibody; SMAD9 Antibody (Center); Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9; SMAD9; MADH6; MADH9; anti-SMAD9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
200-228
Sequence Length
430
Applicable Applications for anti-SMAD9 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.)
WB (Western Blot)
(SMAD9 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).)
WB (Western Blot)
(SMAD9 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).)
Related Product Information for anti-SMAD9 antibody
The protein encoded by this gene is a member of the SMAD
family, which transduces signals from TGF-beta family members. The
encoded protein is activated by bone morphogenetic proteins and
interacts with SMAD4. Two transcript variants encoding different
isoforms have been found for this gene.
family, which transduces signals from TGF-beta family members. The
encoded protein is activated by bone morphogenetic proteins and
interacts with SMAD4. Two transcript variants encoding different
isoforms have been found for this gene.
Product Categories/Family for anti-SMAD9 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Liu, Y., et al. FASEB J. 23(7):2299-2306(2009)
Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009)
Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009)
Hoover, L.L., et al. Sci Signal 1 (46), PE48 (2008) :
Liu, Y., et al. FASEB J. 23(7):2299-2306(2009)
Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009)
Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009)
Hoover, L.L., et al. Sci Signal 1 (46), PE48 (2008) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52493
NCBI Official Full Name
mothers against decapentaplegic homolog 9 isoform a
NCBI Official Synonym Full Names
SMAD family member 9
NCBI Official Symbol
SMAD9
NCBI Official Synonym Symbols
PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
NCBI Protein Information
mothers against decapentaplegic homolog 9
UniProt Protein Name
Mothers against decapentaplegic homolog 9
UniProt Gene Name
SMAD9
UniProt Synonym Gene Names
MADH6; MADH9; MAD homolog 9; Mothers against DPP homolog 9; SMAD 9; Smad9
UniProt Entry Name
SMAD9_HUMAN
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Product Notes
The SMAD9 smad9 (Catalog #AAA283861) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 200-228. The SMAD9 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SMAD9 smad9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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