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IF (Immunofluorescence)
(Immunofluorescence staining of KLH-SMCCA825+KLH-SMCCA1177 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA825+KLH-SMCCA1177 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit SMAD9 Polyclonal Antibody | anti-SMAD9 antibody
Anti-SMAD9 Antibody, Rabbit Polyclonal
Reactivity
Human, Mouse, Rat(Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
SMAD9, Antibody; Anti-SMAD9 Antibody, Rabbit Polyclonal; SMAD family member 9; Anti-MADH6 Antibody; Anti-MADH9 Antibody; Anti-PPH2 Antibody; Anti-SMAD8 Antibody; Anti-SMAD8A Antibody; Anti-SMAD8B Antibody; anti-SMAD9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SMAD9 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the center region of the Human SMAD9
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human SMAD9, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of KLH-SMCCA825+KLH-SMCCA1177 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA825+KLH-SMCCA1177 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of KLH-SMCCA825+KLH-SMCCA1177 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA825+KLH-SMCCA1177 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohistochemisry)
(Immunochemical staining of rat D9 in rat kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of rat D9 in rat kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse SMAD9 in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse SMAD9 in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human SMAD9 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human SMAD9 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-SMAD9 antibody
SMAD9 is one of the important transcription factors in the BMP/SMAD pathway and is involved in goose follicular initiation. SMAD9 is differentially expressed in goose follicles and acts as a key player in the control over goose follicular selection. Smad1, Smad5, and Smad9 (also known as Smad8) are activated by phosphorylation by bone morphogenetic protein (BMP)-bound type I receptor kinases. Smad9 is a new type of transcriptional regulator in BMP signaling. smad1 and smad9 act redundantly to each other downstream of smad5 to mediate ventral specification and to regulate embryonic myelopoiesis. Functional regions analysis of Smad9 indicated that SMAD9 protein is highly conserved in MH1 and MH2 domains, and the connection area is the highly variable region. miR-203a-3p.1 may serve an important role in the osteogenic differentiation of multiple myeloma (MM)-mesenchymal stem cells (MSCs) by regulating Smad9 expression.
References
Yu D, et al. (2019) A potential role for smad9 in goose follicular selection through regulation of mrna levels of luteinizing hormone receptor. Theriogenology 135 204-212.
Wei CY, et al. (2014) Transcriptional factors smad1 and smad9 act redundantly to mediate zebrafish ventral specification downstream of smad5. J Biol Chem 289 (10): 6604-6618.
Tsukamoto S, et al. (2014) Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling. Sci Rep 4 7596.
Fan FY, et al. (2019) Mir-203a-3p.1 is involved in the regulation of osteogenic differentiation by directly targeting smad9 in mm-mscs. Oncol Lett 18 (6): 6339-6346.
Wei CY, et al. (2014) Transcriptional factors smad1 and smad9 act redundantly to mediate zebrafish ventral specification downstream of smad5. J Biol Chem 289 (10): 6604-6618.
Tsukamoto S, et al. (2014) Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling. Sci Rep 4 7596.
Fan FY, et al. (2019) Mir-203a-3p.1 is involved in the regulation of osteogenic differentiation by directly targeting smad9 in mm-mscs. Oncol Lett 18 (6): 6339-6346.
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Product Notes
The SMAD9 (Catalog #AAA258754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMAD9 Antibody, Rabbit Polyclonal reacts with Human, Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SMAD9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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