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product-image-AAA98608_SC15.jpg Standard Curve (Sample)

Human Netrin receptor UNC5C ELISA Kit | UNC5C elisa kit

Human Netrin receptor UNC5C ELISA Kit

Reactivity
Human
Synonyms
Netrin receptor UNC5C; N/A; Human Netrin receptor UNC5C ELISA Kit; UNC5C/UNC5H3/netrin receptor UNC5C/Protein unc-5 homolog 3/Protein unc-5 homolog C/unc-5 homolog 3/unc-5 homolog C (C. elegans)/UNC5H3unc5 (C.elegans homolog) c; UNC5C elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of UMOD. No significant cross-reactivity or interference between UMOD and analogues was observed.
Sequence Length
931
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UMOD were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UMOD were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

product-image-AAA98608_SC15.jpg Standard Curve (Sample)
Related Product Information for UNC5C elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-UMOD antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UMOD antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UMOD amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of UMOD can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,135 Da
NCBI Official Full Name
netrin receptor UNC5C
NCBI Official Synonym Full Names
unc-5 netrin receptor C
NCBI Official Symbol
Unc5c
NCBI Protein Information
netrin receptor UNC5C
UniProt Protein Name
Netrin receptor UNC5C
UniProt Gene Name
Unc5c
UniProt Entry Name
UNC5C_RAT

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Product Notes

The Human UNC5C unc5c (Catalog #AAA98608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA98608 ELISA Kit recognizes Human UNC5C. It is sometimes possible for the material contained within the vial of "Netrin receptor UNC5C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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