IF (Immunofluorescence)
(ICC/IF analysis of NPM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human NPM Monoclonal Antibody | anti-NPM antibody
NPM antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
NPM, Antibody; NPM antibody; nucleophosmin (nucleolar phosphoprotein B23,numatrin),B23; NPM; MGC104254; nucleolar phosphoprotein B23; numatrin; nucleophosmin/nucleoplasmin family; member 1; anti-NPM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
5E3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
294
Applicable Applications for anti-NPM antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human NPM (81-294aa) purified from E. coli
Clone Note
Anti-human NPM mAb, clone 5E3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of NPM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of NPM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NPM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry)
(This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.)
IHC (Immunohiostchemistry)
(This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.)
WB (Western Blot)
(The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-NPM antibody
Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.
Product Categories/Family for anti-NPM antibody
References
Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711; Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,400 Da
NCBI Official Full Name
nucleophosmin isoform 1
NCBI Official Synonym Full Names
nucleophosmin 1
NCBI Official Symbol
NPM1
NCBI Official Synonym Symbols
B23; NPM
NCBI Protein Information
nucleophosmin
UniProt Protein Name
Nucleophosmin
UniProt Gene Name
NPM1
UniProt Synonym Gene Names
NPM; NPM
Similar Products
Product Notes
The NPM npm1 (Catalog #AAA47993) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NPM antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPM can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NPM npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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