Standard Curve (Sample)
(Fig.1. Human Pancreas transcription factor 1 subunit alpha (PTF1A) Standard Curve.)
Human Pancreas Transcription Factor 1 Subunit alpha (PTF1A) ELISA Kit | PTF1A elisa kit
Human Pancreas Transcription Factor 1 Subunit alpha (PTF1A) ELISA Kit
Gene Names
PTF1A; p48; PACA; PAGEN2; bHLHa29; PTF1-p48
Reactivity
Human
Synonyms
Pancreas Transcription Factor 1 Subunit alpha (PTF1A); N/A; Human Pancreas Transcription Factor 1 Subunit alpha (PTF1A) ELISA Kit; PTF1A; PTF1-p48; bHLHa29; bHLH transcription factor p48; class bHLH protein PTF1A; exocrine pancreas-specific transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; pancreas transcr; PTF1A elisa kit
Reactivity
Human
Specificity
Human Pancreas transcription factor 1 subunit alpha (PTF1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTF1A. No significant cross-reactivity or interference between Human PTF1A and analogues was observed.
Sequence Length
328
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pancreas transcription factor 1 subunit alpha (PTF1A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pancreas transcription factor 1 subunit alpha (PTF1A) Standard Curve.)
Related Product Information for PTF1A elisa kit
Description: This Human Pancreas transcription factor 1 subunit alpha (PTF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PTF1A in samples. An antibody specific for PTF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pancreas transcription factor 1 subunit alpha is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
Background: Pancreas transcription factor 1 subunit alpha is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pancreas transcription factor 1 subunit alpha
NCBI Official Synonym Full Names
pancreas associated transcription factor 1a
NCBI Official Symbol
PTF1A
NCBI Official Synonym Symbols
p48; PACA; PAGEN2; bHLHa29; PTF1-p48
NCBI Protein Information
pancreas transcription factor 1 subunit alpha
UniProt Protein Name
Pancreas transcription factor 1 subunit alpha
UniProt Gene Name
PTF1A
UniProt Synonym Gene Names
BHLHA29; PTF1P48; bHLHa29; PTF1-p48
UniProt Entry Name
PTF1A_HUMAN
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Product Notes
The Human PTF1A ptf1a (Catalog #AAA331254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331254 ELISA Kit recognizes Human PTF1A. It is sometimes possible for the material contained within the vial of "Pancreas Transcription Factor 1 Subunit alpha (PTF1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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