Rabbit PTF1A Polyclonal Antibody | anti-PTF1A antibody

Anti-PTF1A Antibody, Rabbit Polyclonal

Cat. Num. AAA254293

• Gene Names: PTF1A; bHLHa29; PTF1-p48
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PTF1A, Antibody; Anti-PTF1A Antibody, Rabbit Polyclonal; PTF1A Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-bHLHa29 Antibody; Anti-PTF1-p48 Antibody; anti-PTF1A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PTF1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PTF1A antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human PTF1A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PTF1A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of PTF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PTF1A polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)

WB (Western Blot)

(Anti-PTF1A rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-PTF1A antibody

Homozygous truncating mutations in the helix-loop-helix transcription factor PTF1A are a rare cause of pancreatic and cerebellar agenesis. The correlation of Ptf1a dosage with pancreatic phenotype in a mouse model suggested the possibility of finding hypomorphic PTF1A mutations in patients with pancreatic agenesis or neonatal diabetes but no cerebellar phenotype. Ptf1a-deficient acinar cells are dramatically sensitized to KRAS transformation, and reduced Ptf1a greatly accelerates development of invasive PDAC.

NCBI and Uniprot Product Information

• NCBI GI #: 30039710
• NCBI GeneID: 256297
• NCBI Accession #: NP_835455.1
• NCBI GenBank Nucleotide #: NM_178161.2
• UniProt Accession #: Q7RTS3; Q9HC25
• Molecular Weight: 34,970 Da
• NCBI Official Full Name: pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: PTF1A
• NCBI Official Synonym Symbols: bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha; class II bHLH protein PTF1A; bHLH transcription factor p48; class A basic helix-loop-helix protein 29; pancreas-specific transcription factor 1a; exocrine pancreas-specific transcription factor p48; p48 DNA-b
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• UniProt Gene Name: PTF1A
• UniProt Synonym Gene Names: BHLHA29; PTF1P48; bHLHa29; PTF1-p48
• UniProt Entry Name: PTF1A_HUMAN