Standard Curve (Sample)
(Fig.1. Human Paraplegin (SPG7) Standard Curve.)
Human Paraplegin (SPG7) ELISA Kit | SPG7 elisa kit
Human Paraplegin (SPG7) ELISA Kit
Gene Names
SPG7; CAR; PGN; CMAR; SPG5C
Reactivity
Human
Synonyms
Paraplegin (SPG7); N/A; Human Paraplegin (SPG7) ELISA Kit; SPG7; CAR; CMAR; FLJ37308; MGC126331; MGC126332; PGN; SPG5C; cell adhesion regulator; cell matrix adhesion regulator; paraplegin; isoform 1; spastic paraplegia 7; SPG7 elisa kit
Reactivity
Human
Specificity
Human Paraplegin (SPG7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPG7. No significant cross-reactivity or interference between Human SPG7 and analogues was observed.
Sequence Length
795
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Paraplegin (SPG7) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Paraplegin (SPG7) Standard Curve.)
Related Product Information for SPG7 elisa kit
Description: This Human Paraplegin (SPG7) ELISA Kit employs a two-site sandwich ELISA to quantitate SPG7 in samples. An antibody specific for SPG7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPG7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPG7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPG7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Paraplegin (SPG7) encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
Background: Paraplegin (SPG7) encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
paraplegin isoform 1
NCBI Official Synonym Full Names
SPG7 matrix AAA peptidase subunit, paraplegin
NCBI Official Symbol
SPG7
NCBI Official Synonym Symbols
CAR; PGN; CMAR; SPG5C
NCBI Protein Information
paraplegin
UniProt Protein Name
Paraplegin
UniProt Gene Name
SPG7
UniProt Synonym Gene Names
CAR; CMAR; PGN
UniProt Entry Name
SPG7_HUMAN
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Product Notes
The Human SPG7 spg7 (Catalog #AAA330744) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330744 ELISA Kit recognizes Human SPG7. It is sometimes possible for the material contained within the vial of "Paraplegin (SPG7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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