IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SPG7 protein using SPG7 antibody)
Mouse SPG7 Monoclonal Antibody | anti-SPG7 antibody
SPG7 antibody
Gene Names
SPG7; CAR; PGN; CMAR; SPG5C
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
SPG7 antibody was purified by affinity chromatography.
Synonyms
SPG7, Antibody; SPG7 antibody; Monoclonal SPG7; Anti-SPG7; CAR; CMAR; FLJ37308; MGC126331; MGC126332; PGN; SPG5C; SPG7; SPG 7; SPG-7; anti-SPG7 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1C12
Purity/Purification
SPG7 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
732
Applicable Applications for anti-SPG7 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7.
Cross-Reactivity
Human, Mouse
Immunogen
SPG7 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 prodduced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SPG7 protein using SPG7 antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SPG7 protein using SPG7 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SPG7 protein (Right) or empty vector (Left) detected with SPG7 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SPG7 protein (Right) or empty vector (Left) detected with SPG7 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SPG7 protein (red) or empty vector (blue) stained using SPG7 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SPG7 protein (red) or empty vector (blue) stained using SPG7 antibody)
Related Product Information for anti-SPG7 antibody
Mouse monoclonal SPG7 antibody
Product Categories/Family for anti-SPG7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
53,940 Da
NCBI Official Full Name
SPG7 protein, partial
NCBI Official Synonym Full Names
spastic paraplegia 7 (pure and complicated autosomal recessive)
NCBI Official Symbol
SPG7
NCBI Official Synonym Symbols
CAR; PGN; CMAR; SPG5C
NCBI Protein Information
paraplegin
UniProt Protein Name
Paraplegin
UniProt Gene Name
SPG7
UniProt Synonym Gene Names
CAR; CMAR; PGN
UniProt Entry Name
SPG7_HUMAN
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Product Notes
The SPG7 spg7 (Catalog #AAA106650) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SPG7 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the SPG7 spg7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPG7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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