Human PD-L1/CD274 ELISA Kit | PD-L1/CD274 elisa kit

Human PD-L1/CD274 ELISA Kit

Cat. Num. AAA258323

• Reactivity: Human
• Synonyms: PD-L1/CD274; N/A; Human PD-L1/CD274 ELISA Kit; PD-L1 ELISA Kit, Human; CD274 molecule; B7-H ELISA Kit, Human; B7-H1 ELISA Kit, Human; B7H1 ELISA Kit, Human; PDCD1L1 ELISA Kit, Human; PDCD1LG1 ELISA Kit, Human; PDL1 ELISA Kit, Human; PD-L1/CD274 elisa kit

• Reactivity: Human
• Specificity: For the quantitative determination of Human PD-L1/CD274 concentration in serum, cell culture supernates and plasma.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Serum, Cell supernatant, Plasma
• Detection Range: 10.94-700pg/mL
• Sensitivity: 4.64pg/mL
• Conjugation: HRP
• Interference: Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human PD-L1 control were assayed for interference. No significant interference was observed.
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural human PD-L1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for PD-L1/CD274 elisa kit

This PD-L1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PD-L1 protein in Serum, Cell supernatant, Plasma. It contains recombinant Human PD-L1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

References

Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.