Rabbit PD L1 Polyclonal Antibody | anti-PD L1 antibody

PD L1 Antibody (C-Term)

Cat. Num. AAA290915

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human; Predicted: Human
• Applications: ELISA, Western Blot
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: PD L1, Antibody; PD L1 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; anti-PD L1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This PD L1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 252-290 amino acids from the human region of human PD L1.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 176

• Applicable Applications for anti-PD L1 antibody: ELISA, WB (Western Blot)
• Function: Plays a critical role in induction andmaintenance of immune tolerance to self(PubMed: , PubMed: 28813417,PubMed: ). As a ligand for the inhibitory receptor PDCD1/PD-1,modulates the activation threshold of T-cells and limits T-cell effector response(PubMed: , PubMed: 28813417,PubMed: ). Through ayet unknown activating receptor, maycostimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed: ).
• Cellular Location: Cell membrane; Single-pass type I membran eprotein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-passtype I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. [Isoform 2]: Endomembrane system; Single-pass type I membrane protein
• Tissue Location: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressedin the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

WB (Western Blot)

(Anti-PD L1 Antibody (C-Term) at 1:1000 dilution + Human heart whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilutionLane 1: Human heart lysateLane 2: Human skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PD L1 antibody

Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Product Categories/Family for anti-PD L1 antibody

Primary Antibodies; Immunology

References

Dong H.,et al.Nat. Med. 5:1365-1369(1999). Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000). He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005). Chi X.-Y.,et al. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NM_001267706.1
• UniProt Accession #: Q9NZQ7; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
• Molecular Weight: 33275
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1